There could be a problem with the floppy drive itself or you need to create a new boot disk.

If it prompts "Please insert disk" the floppy drive might be good.

NOT TESTED, USE AT OWN RISK

YOU MUST HAVE A 3.5" FLOPPY DISK DRIVE ON YOUR COMPUTER

To create a new boot disk:

1. Download.

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https://preview.redd.it/sw1o2rxaf13b1.png?width=454&format=png&auto=webp&v=enabled&s=22340afbe8c09343754ca347def21ff9b46d0e75

1. Unzip the zip file to a folder.

2. Plug in your External 3.5 inch Floppy Drive.

3. READ THE README.TXT FILE FIRST.

4. Run epsos.exe.

5. Follow prompts and instructions.

I can't test the software without the Ensoniq EPS so USE AT YOUR OWN RISK

Good luck and happy playing